產(chǎn)品詳情
簡單介紹：
重組鼠蛋白C10 / CCL6蛋白與其它公司提供的重組蛋白不同，rMuC10/CCL6蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠蛋白C10 / CCL6蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠蛋白C10 / CCL6蛋白
| Synonyms | Small-inducible Cytokine A6 |
| Species | Murine |
| Accession | P27784 |
| GeneID | 20305 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠蛋白C10 / CCL6蛋白Approximately 10.7 kDa, a single non-glycosylated polypeptide chain containing 95 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠蛋白C10 / CCL6蛋白Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, pH 8.0, 500 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rMuC10/CCL6 as determined by LAL method. |
| Reconstitution | 重組鼠蛋白C10 / CCL6蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠蛋白C10 / CCL6蛋白 | |
| Reference |
1. Berger MS, Kozak CA, Gabriel A, et al. 1993. DNA Cell Biol. 12:839-47. 2. Orlofsky A, Berger MS, Prystowsky MB. 1991. Cell Regul. 2:403-12. 3. Ma B, Zhu Z, Homer RJ, et al. 2004. J Immunol. 172:1872-81. |
| Background | Chemokine (C-C motif) ligand 6 (CCL6), encoded by the gene CCL6, is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. Murine CCL6 is expressed in myelopoietic bone marrow cultures when stimulated with GM-CSF, M-CSF, IL-3 or IL-4 and has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. It signals primarily through the CCR1 receptor. CCL6 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. The C10 contains the four highly conserved cysteine residues present in CC chemokines. |
