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產品名稱：重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白
產品型號：rMuEotaxin/CCL11
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白與其它公司提供的重組蛋白不同，rMuEotaxin/CCL11蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白

Synonyms Small-inducible Cytokine A11

Species Murine

Accession P48298

GeneID 20292

Source Escherichia coli.

Molecular Weight 重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rMuEotaxin/CCL11 as determined by LAL method.

Reconstitution 重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白

SDS-PAGE

Reference 1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12.
2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7.
3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56.
4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5.

Background Murine CCL11 is belonging to the CC chemokine family. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Murine CCL11 is approximately 63 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor.

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Synonyms	Small-inducible Cytokine A11
Species	Murine
Accession	P48298
GeneID	20292
Source	Escherichia coli.
Molecular Weight	重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rMuEotaxin/CCL11 as determined by LAL method.
Reconstitution	重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白
SDS-PAGE
Reference	1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12. 2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7. 3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56. 4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5.
Background	Murine CCL11 is belonging to the CC chemokine family. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Murine CCL11 is approximately 63 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor.

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產品名稱：重組鼠嗜酸性粒細胞趨化因子/ CCL11蛋白