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產(chǎn)品名稱：重組鼠β細胞蛋白
產(chǎn)品型號：rMuBetacellulin
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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簡單介紹：

重組鼠β細胞蛋白與其它公司提供的重組蛋白不同，rMuBetacellulin蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠β細胞蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組鼠β細胞蛋白

Species Murine

Accession Q05928

GeneID 12223

Source Escherichia coli.

Molecular Weight 重組鼠β細胞蛋白Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Quantity 5μg/20μg/1000μg

AA Sequence DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組鼠β細胞蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 10⁸ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rMuBetacellulin as determined by LAL method.

Reconstitution 重組鼠β細胞蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組鼠β細胞蛋白

SDS-PAGE

Reference 1. Miura K, Doura H, Aizawa T, et al. 2002. Biochem Biophys Res Commun. 294:1040-6.
2. Kim HS, Shin HS, Kwak HJ, et al. 2003. FASEB J. 17:318-20.
3. Nakagawa T, Furuta H, Sanke T, et al. 2005. Diabetes Res Clin Pract. 68:188-92.

Background Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family of cytokines that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80% identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant murine beta-cellulin is a heparinbinding protein containing 80 amino acids residues.

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Species	Murine
Accession	Q05928
GeneID	12223
Source	Escherichia coli.
Molecular Weight	重組鼠β細胞蛋白Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.
Quantity	5μg/20μg/1000μg
AA Sequence	DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組鼠β細胞蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 10⁸ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rMuBetacellulin as determined by LAL method.
Reconstitution	重組鼠β細胞蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠β細胞蛋白
SDS-PAGE
Reference	1. Miura K, Doura H, Aizawa T, et al. 2002. Biochem Biophys Res Commun. 294:1040-6. 2. Kim HS, Shin HS, Kwak HJ, et al. 2003. FASEB J. 17:318-20. 3. Nakagawa T, Furuta H, Sanke T, et al. 2005. Diabetes Res Clin Pract. 68:188-92.
Background	Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family of cytokines that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80% identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant murine beta-cellulin is a heparinbinding protein containing 80 amino acids residues.

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產(chǎn)品名稱：重組鼠β細胞蛋白