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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組鼠白介素-21蛋白

  • 產(chǎn)品型號:rMuIL-21
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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簡單介紹:
重組鼠白介素-21蛋白與其它公司提供的重組蛋白不同,rMuIL-21蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白,可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨(dú)有的全自動蛋白純化技術(shù)則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠白介素-21蛋白(全長序列)產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組鼠白介素-21蛋白

Species Murine
Accession Q9ES17
GeneID 60505
Source Escherichia coli.
Molecular Weight Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
Quantity 2μg/10μg/1000μg
AA Sequence 重組鼠白介素-21蛋白HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS
Purity > 98 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.
Physical Appearance 重組鼠白介素-21蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Endotoxin Less than 0.1 EU/μg of rMuIL-21 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage 重組鼠白介素-21蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組鼠白介素-21蛋白
SDS-PAGE

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