產(chǎn)品詳情
簡(jiǎn)單介紹：
重組小鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白與其它公司提供的重組蛋白不同，rMuFGF-9蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組小鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組小鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白
| Synonyms | GAF, HBGF-9 |
| Species | Murine |
| Accession | P54130 |
| GeneID | 14180 |
| Source | Escherichia coli. |
| Molecular Weight | 重組小鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MPLGEVGSYF GVQDAVPFGN VPVLPVDSPV LLNDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS |
| Purity | 重組小鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組小鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 8.5. |
| Endotoxin | Less than 1 EU/μg of rMuFGF-9 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組小鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白 | |
| SDS-PAGE |
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| Reference |
1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9. 2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31. 3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94. |
| Background | Fibroblast growth factor 9 (FGF-9) encoded by the FGF-9 gene is a member of the fibroblast growth factor (FGF) family. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In addition, this protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells and it is a heparin-binding protein. Furthermore, FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Recombinant mouse FGF-9 is synthesized as a 208 a.a. precursor that contains a 3 a.a. signal sequence. Specifically, The mouse FGF-9 shares 99 % a.a. sequence identity with rat FGF-9. |
